python - Image processing approach -
in following greyscale image,i trying identify objects have manually labelled in red. have suggestions of how this? i have attempted use gaussian blur , thresholding cannot exclusively identify these particles. edge detection approach? suggestions welcome. your images suitable target machine learning . i created following probability map using trainable weka segmentation plugin imagej/fiji (taking second image input): you should able objects simple thresholding , possibly subsequent size filtering. you might want try ilastik , free software combines pixel classification , object tracking power of machine learning. edit : this how proceeded using trainable weka segmentation: in settings window, activated more features, set sigma range 4 32, , named classes "objects" , "background": i created freehand line traces, added them respective classes, , clicked on "train classifier". (the creation of feature stack takes...